tImplementing of high strength vinegar fermentation is still the mission of vinegar producers. The aimof this study was to carry out high acidity vinegar fermentation efficiently based on comprehensiveanalysis on bacterial fermentation kinetics characteristics of Acetobacter pasteurianus CICIM B7003-02. Inpractice, semi-continuous vinegar fermentation was optimized with an optimal discharge/charge ratioof 34% of working volume (v/v), which resulted in a proper growth status of Acetobacter and beneficial toacetification. Then, a two-stage aeration protocol was adopted in the vinegar fermentation in line withthe Acetobacter theoretical oxygen demand, by which both vinegar stoichiometric yield and acetificationrate were improved effectively. As the final result, a titer of 93.09 ± 0.24 g/L acetic acid was achieved,the average acetification rate was enhanced to a level of 1.83 ± 0.01 g/L/h, and the vinegar stoichiometricyield was promoted to 93.97 ± 0.16%. The strategy and practice worked out from this study provided avaluable reference for performing large scale vinegar fermentation with higher strength