The
DNA was recovered by ethanol precipitation, dissolved in
Tris-EDTA (10 mM Tris-Cl, 1 HIM EDTA, pH 8.0), and centrifuged
overnight in a cesium gradient. DNA was digested
with Hindlll, separated on a 1% (w/v) agarose gel, and
transferred to a Zeta-probe membrane (Bio-Rad) according to
manufacturer's recommendations.