To study the effect of biocontrol agents on the pathogenic fungal
strains, scanning electron microscopy was done for both treated
and untreated fungal mycelia. The mycelia were taken from
inhibited zone interface area as treated samples and uninhibited
part as control one. Then the mycelia were prefixed with 2% glutaraldehyde
in 20 mM Na–P buffer (pH 6.5) plus 5% dimethyl sulphoxide
(DMSO) for 30 min. After pre-fixation the mycelia were
gently washed with sterilized water and post fixed with osmium
tetraoxide dissolved in 50 mM Na–P buffer (pH 6.5). Mycelia were
then dehydrated in series of alcohol grades starting from 30% to