. Photomicrographs of Enterocytozoon hepatopenaei tubule epithelial cells of the hepatopancreas of Penaeus monodon. (A) H&E-stained smear of hepatopancreatic tissue
showing numerous microsporidian spores (arrows). (B) Fresh preparation of microsporidian spores from a Percoll gradient. (C and D) Hepatopancreatic tissue sections
showing acidophilic, granular inclusions in the cytoplasm of tubule epithelial cells (arrows). (E) Semi-thin section of hepatopancreatic tissue showing early and late
plasmodia (inset a) and mature spores (inset b) in the cytoplasm of tubule epithelial cells. Some spores show unstained spots that represent their concave surfaces at one end
(inset b). A, haematoxylin stain; C, Trichrome stain; D, H&E stain; E, Toluidine blue stain. ePm, early plasmodium; lPm, late plasmodium.
. Photomicrographs of Enterocytozoon hepatopenaei tubule epithelial cells of the hepatopancreas of Penaeus monodon. (A) H&E-stained smear of hepatopancreatic tissue
showing numerous microsporidian spores (arrows). (B) Fresh preparation of microsporidian spores from a Percoll gradient. (C and D) Hepatopancreatic tissue sections
showing acidophilic, granular inclusions in the cytoplasm of tubule epithelial cells (arrows). (E) Semi-thin section of hepatopancreatic tissue showing early and late
plasmodia (inset a) and mature spores (inset b) in the cytoplasm of tubule epithelial cells. Some spores show unstained spots that represent their concave surfaces at one end
(inset b). A, haematoxylin stain; C, Trichrome stain; D, H&E stain; E, Toluidine blue stain. ePm, early plasmodium; lPm, late plasmodium.
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