During the fermentation process 1.5% glucose and 0.45% xylose were supplemented into the enzymatic OMSW hydrolysate medium. The results showed that ethanol production using the X, G, S.C. yeast strains was of 3.08, 0.54, 0.58 mg ethanol/g utilized glucose/h), respectively. This indicated that the isolated strain X was superior compared with the other two strains.