Evaluation of antibacterial activity of phytosynthesized
calcium phosphate nanoparticles
The phytosynthesized calcium phosphate nanoparticles
were tested for antibacterial activity by Kirby–Bauer disk
diffusion method (Bauer et al. 1966). The culture of
Streptococcus mutans (MTCC-890) was procured from
culture collection center IMTECH, Chandigarh, while
Staphylococcus aureus (ATCC-25923) and Escherichia
coli (ATCC-39403) were obtained from American Type
Culture Collection Center, USA. S. aureus and E. coli were
grown on Mueller–Hinton agar medium, while brain heart
infusion agar medium was used for S. mutans. The disk
diffusion was performed by placing four different types of
disks including control, calcium phosphate nanoparticles,
standard antibiotic (erythromycin) and calcium phosphate
nanoparticles with standard antibiotic on the surface of the
agar in plates and incubated for 24 h at 37 C. Zones of
inhibition were measured by the Hi-Media scale (Birla
et al. 2009). The assays were performed in triplicate and
mean was calculated.