For the DPPH method, a 100 mL aliquot of each dilution was
added to 3.9 mL of DPPH radical (Sigma–Aldrich, St. Louis, MO,
USA), and the reading made at 515 nm in an Ultrospec 3100 pro
spectrophotometer (Amersham Pharmacia Biotech, Cambridge,
UK) after 30 min, using methanol as the blank. For the ABTS
method, an aliquot of 30 mL of each dilution was added to 3.0 mL of
ABTS radical (Sigma–Aldrich), and the reading made in the
spectrophotometer at 734 nm after 6 min of reaction, using
ethanol as the blank and a standard curve prepared using Trolox
(Sigma–Aldrich). The analyses of the extract were carried out in
triplicate and the results presented in g or mL of sample/g of DPPH
or in mM of Trolox equivalents (TE)/g or mL of sample.