procedure
preparation : prepare sample 10 g. homogenize with n-hexane 20 mland filter
condition : condition the Vertipak Silica Tubes 500 mg/3ml with n hexane 6 ml
Loading : load the sample through the SPE column
Washing : wash the SPE column with n hexane 1 ml
Elute : Elute the SPE columnwith n hexane/ethyl acelate
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Chloramphenicol in Chicken Using Vertipak c18
General Information : Chloramphenicol is an antibiotic which used in both human and veterinary medicine .
Chloramphenicol is biosynthesised by the soil organism Streptomyces venezuelae and several other actinomycetes and can produce from chemical synthesis
Chloramphenicol has been banned for use in food producing animals the EU and many other countries,because of concerns over its genotoxicity
embryo-and fetoxicity carcinogenic potential and the lack of a dose response relationship for aplastic anaemia
EU requiring testing of aquaculture and poultry products for export to the EU in order to protect the european consumer from imported products containing potentially harmful residues of Chloramphenicol
Solid Phase extraction can be used for cleanup of Chloramphenicol in chicken tissue analysis by LC/MS/MS