4. Results and discussionThe microfluidic culture-based sensor consists of amicrochannel fabricated inCOCcontaining paraffin islands usedfor bating and culturing bacteria. The final device formed fromtwo 1-mm thick COC substrates had dimensions approximately2 cm×3 cm (Fig. 6). The microchannel was 1mm in width,15mm in length, and 110m in height, while input and outputports were 600m in diameter. The three paraffin islands insidethe microchannel were spaced at 2 mm,each being 800 mwide,1.5mm long, and 5m thick. COC is a relatively new thermoplasticpolymer that has been gaining popularity [33–35] andwasselected as substrate material in this work due to its excellentphysical and chemical properties. COC is more chemically inertthan most thermoplastics and exhibits very low (<0.01%) waterabsorption [33,35]. COC has excellent optical properties (e.g.,high transmission >350 nm) [36], and thus is an ideal materialfor our fluorescence-based detection method. High transmissionis also important since we use a UV-cured adhesive to sealmicrochannels at ambient conditions. Most importantly, COCoffers superior adhesion to paraffin.
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