Thus, while PCR was sufficient for the identification of C. glabrata and C. guilliermondii,
the RFLP technique using the restriction enzyme Msp I was used for the identification of additional four Candida species:C. albicans, C. tropicalis, C. krusei, and C. parapsilosis
(Fig. 3a and b). There is no recognition site for this enzyme within ITS region of C. parapsilosis; therefore, the same band (520 bp) was obtained before and after digestion with Msp I
enzyme. Repeated testing of the isolates was found to yield consistent results.