2.The TRNG isolates carrying the American or the Dutch type
plasmids. These two plasmid types carry thetetM genes that
only show minor differences in DNA sequence. Thus, a primer
set binding at the same region for both plasmid types and produce an 841 bp product in both types. However, American
type contains only oneHinfI restriction site while the Dutch
type contains two sites. After cut withHinfI enzyme, product
sizes of the American type are 748 and 93 bp while the product sizes of Dutch type are 572, 176, and 93 bp.