reveals the effects of HPOX nanoparticles
on the generation of H2O2-mediated ROS. Cells given DCFHeDA
only showed very weak fluorescence. A strong fluorescence was
observed with cells treated with exogenous H2O2 because
DCFHeDA was oxidized to fluorescent DCF by the exogenous H2O2
and H2O2-mediated oxidants. Pretreatment of free HBA reduced
the H2O2-induced fluorescence in a dose-dependent fashion. POX
nanoparticles also showed reduction of H2O2-induced fluorescence
almost the same extent as 0.5 mM of HBA. HPOX nanoparticles
exhibited a significantly reduction in H2O2-induced fluorescence in
a dose-dependent manner. Pretreatment with 50 mg of HPOX
nanoparticles resulted in more than 80% reduction in H2O2-
induced fluorescence.