ABSTRACT.A new species, AdelphamargaritaWillmott & Hall, new species, is described from Andean cloud forest habitats from southern Ecuador to Bolivia. AdelphamargaritagarleppiWillmott, new subspecies, is described for southern Peruvian and Bolivian individuals, which differ from the nominate subspecies in having complete orange postdiscal bands on the dorsal surface. The new species differs from related species in the Adelpha serpa group in wing pattern, DNA sequence data and habitat.