Ozone represents a potent antimicrobial compound that is already proposed as a possible sanitizing agent,
especially for surface decontamination of fruits and vegetables. The main objective of this study was to evaluate
the effect of ozone, either in aqueous or gaseous form, on wine grapemycobiota and its impact during spontaneous
and inoculated fermentations. Gaseous (32±1 μL/L, 12 and 24 h) and aqueous (5±0.25mg/L, 6 and 12min)
ozone were tested as sanitizing treatments. A multiphasic approach was used employing culture-dependent
(traditional plate counts) and -independent techniques, based on DNA and RNA amplification (PCR-denaturing
gradient gel electrophoresis [DGGE] and reverse transcription PCR [RT-PCR]-DGGE), respectively. Microbiological
analysis data highlighted a reduction of N0.5 Log CFU/mL of the total yeasts present on grape berry surfaces after
ozone treatments, mainly due to the reduction of apiculate yeasts. The chemical analysis of the wines, produced
from the treated grapes, showed higher acetic acid content in the untreated spontaneous fermentations
(0.52 g/L) compared to the treated (ranged from 0.16 to 0.38 g/L), while all fermentation-inoculated wines
contained higher amounts of pleasant volatile compounds.
Ozone represents a potent antimicrobial compound that is already proposed as a possible sanitizing agent,especially for surface decontamination of fruits and vegetables. The main objective of this study was to evaluatethe effect of ozone, either in aqueous or gaseous form, on wine grapemycobiota and its impact during spontaneousand inoculated fermentations. Gaseous (32±1 μL/L, 12 and 24 h) and aqueous (5±0.25mg/L, 6 and 12min)ozone were tested as sanitizing treatments. A multiphasic approach was used employing culture-dependent(traditional plate counts) and -independent techniques, based on DNA and RNA amplification (PCR-denaturinggradient gel electrophoresis [DGGE] and reverse transcription PCR [RT-PCR]-DGGE), respectively. Microbiologicalanalysis data highlighted a reduction of N0.5 Log CFU/mL of the total yeasts present on grape berry surfaces afterozone treatments, mainly due to the reduction of apiculate yeasts. The chemical analysis of the wines, producedfrom the treated grapes, showed higher acetic acid content in the untreated spontaneous fermentations(0.52 g/L) compared to the treated (ranged from 0.16 to 0.38 g/L), while all fermentation-inoculated winescontained higher amounts of pleasant volatile compounds.
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