Transplantation of plantlet to soil was observed. Established
rate on the mountainous region of plantlets induced from
rhizome explant segments has reached 95%. The diagram of
tissue culture propagation of R. coreanum rhizome performed
in this study is shown in Fig. 1. Different carbon sources
including sucrose and sugar were best for anthraquinone yields
in cell suspension cultures of various plants (Zenk et al., 1975;
Suzuki et al., 1984). Sucrose is commonly the most favorable
carbon source for cell growth (Farzami Sepehr and
Ghorbanli, 2002). In addition, both increasing levels of
myoinositol and vitamin free medium suppressed anthraquinone
formation, but the addition of increasing concentrations
of vitamins promoted callus growth (Farzami Sepehr and
Ghorbanli, 2002). A comparison of yield and content of
anthraquinone in crude drug processed from R. coreanu