Three different versions of the PRSV BRcp gene were
amplified and cloned. The primers 5'-A TCA TTCCA TGGCT
GTGGATGCTGGTTTG-3' and 5'-AGCTAACCA TGGGGT
GAAACAGGGTCG-3' were used as 5’-end and 3’-end
primers, respectively , to amplify the Translatable Short (TS)
version, the primers 5'-A TCATTCCA TGGTCCAAGAATGA
AGCTGT-3' and 5'-AGCTAACCA TGGGGTGAAACAGG
GTCG-3' to amplify the Untranslatable Medium (UM)
version, and the primers 5'-A TCA TTCCA TGGGCGTGTTC
CATGAATCAA-3' and 5'-AGCTAACCA TGGGCGAGTAT
TCAGTTGCGC-3' to amplify the Translatable Long (TL)
version