Microorganisms and inoculums
Five microorganisms, Trichoderma reesei Rut-C30
(ATCC 56765), Trichoderma reesei QM9414 (ATCC
26921), Trichoderma sp. IPT778, Trichoderma harzianum
rifai IPT821 - the latter two isolated in a sugar cane cultivation
area, SP, Brazil - and Myceliophthora thermophilaM77, recently isolated through an environmental program
for biodiversity preservation in the State of São Paulo
(BIOTA FAPESP), were maintained in stock on a solid medium
made of wheat bran and sugarcane bagasse
(20:80 w/w). One gram of the stock medium containing the
spores of each microorganism was suspended in 100 mL of
NaCl solution 0.9% (w/w) and this suspension was applied
as the inoculum for the SSC.