Radical scavenging activity (RSA)The ability of PJ to scavenge 2,2-diphenyl-1-picryl hydrazyl(DPPH) radical was measured following the procedure describedby Karioti et al. (2004) with slight modifications (Fawole et al.,2012). In Eppendorf tubes, PJ extract (15 l) was mixed with735 l methanol and 0.1 mM solution of DPPH (750 l) dis-solved in methanol. The mixture was incubated for 30 min in thedark at room temperature before measuring the absorbance at517 nm using a UV–visible spectrophotometer (Thermo ScientificTechnologies, Madison, Wisconsin). The RSA was determined byascorbic acid standard curve (0–2000 M). The results were pre-sented as micro molar ascorbic acid (AA) equivalent per millilitreof crude pomegranate juice (M AAE/ml PJ).