bsa readily dissolves in water and forms a colorless solutions.dissolved bsa reacts with coomassie blue g-250 to form aa blue colored addition product. in this lab you will: a) mix known concentration of bsa dissolved in buffer with coomassie blue g-250 b) measure the absorbance of the reulting blue colored solution and c) plot the measured absorbance of the protein-dye conjugate versus the known concentration of bsa in that sample. the resulting graph will be the protein standard curve that you will then use to determine the amount of protein in your samples of unknown protein concentration