DNA molecular markers, such as restriction fragment length polymorphism(RFLP) (Castle ot al.. 1987 xu et al., 1997 and randomly amplified polymorphic DNA(RAPD) (zhang and Molina, 1995), confer reliable and feasible alternatives to the morphological markers and have been developed for studies of genetic divesity and strain genotyping in mushrooms. Although advantageous in some aspects, these markers bear several limitations in practice. Genotyping RFLP markers requires a large amount of DNA and, because designed probe hybridizes with only a specific DNA region, it yields limited marker information, whilst genotyping RAPD markers is not always reproducible(Rogot and Hoisington, 1993)