vanillic acid (Figure 1G) were detected, and the main
product was instead vanillyl alcohol (Figure 1F). The high-
est yield of vanillyl alcohol at the end of the cultivation
was 80% and was found in cultures with an initial vanillin
concentration of 0.5 mM (Table 2). Most of the vanillin
had been reduced to vanillyl alcohol also in cultures with
an initial vanillin concentration of 2.5 mM, but at higher
vanillin concentrations no vanillyl alcohol was detected.
The results of experiments with 4-hydroxybenzoic acid
are shown in Figure 5 and in Table 1. In the concentration
range studied, 0.5-10 mM, 4-hydroxybenzoic acid did not
show any clear negative effect on the glucose consump-
tion, the culture pH, or the cell viability of
G. xylinus
(Figure 5A-C). The concentration of 4-hydroxybenzoic
acid did not change significantly during the cultivation
(Figure 5D). The yield of BC slightly decreased as the
concentration of 4-hydroxybenzoic acid increased (Table 1).
The lowest yield of BC, 5.0 g/L, was obtained in medium
with 10 mM 4-hydroxybenzoic acid (Table 1B). The BC
yield on consumed glucose also declined with increasing
concentrations of 4-hydroxybenzoic acid, which means that
the cells to an increasing degree utilized glucose for other
purposes than production of BC when the concentration of
the acid increased.
vanillic acid (Figure 1G) were detected, and the main
product was instead vanillyl alcohol (Figure 1F). The high-
est yield of vanillyl alcohol at the end of the cultivation
was 80% and was found in cultures with an initial vanillin
concentration of 0.5 mM (Table 2). Most of the vanillin
had been reduced to vanillyl alcohol also in cultures with
an initial vanillin concentration of 2.5 mM, but at higher
vanillin concentrations no vanillyl alcohol was detected.
The results of experiments with 4-hydroxybenzoic acid
are shown in Figure 5 and in Table 1. In the concentration
range studied, 0.5-10 mM, 4-hydroxybenzoic acid did not
show any clear negative effect on the glucose consump-
tion, the culture pH, or the cell viability of
G. xylinus
(Figure 5A-C). The concentration of 4-hydroxybenzoic
acid did not change significantly during the cultivation
(Figure 5D). The yield of BC slightly decreased as the
concentration of 4-hydroxybenzoic acid increased (Table 1).
The lowest yield of BC, 5.0 g/L, was obtained in medium
with 10 mM 4-hydroxybenzoic acid (Table 1B). The BC
yield on consumed glucose also declined with increasing
concentrations of 4-hydroxybenzoic acid, which means that
the cells to an increasing degree utilized glucose for other
purposes than production of BC when the concentration of
the acid increased.
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