The beta-tubulin gene could not be detected in samples from uninfected wheat. However detection was possible in all samples taken after
inoculation with F. graminearum isolate IFA 191. There was a distinct gradual increase in gene copies measured, corresponding to the fungal growth during
the infection of the wheat spike (Fig. 1). Detection was possible from the first day post inoculation, while first symptoms of the infection (water soaked spots)
appeared 7–10 days after inoculation (see Fig. 1). Data of all aliquots, dilution steps and replicates is included in Fig. 1. The method developed in this study allows a fast,
species-specific identification and quantitation of plant-infections by F. graminearum
at very early