2.3 Production of chitosan-agarose hydrogel
For chitosan-agarose hydrogel production, different percentages of deacetylated chitosan (from 0.75% to 2.5%), previously filtered with a 0.22 micro m filter, were initially dissolved in 1% acetic acid solution. Then, agarose powder was added to the chitosan solution, under stirring at 50 degreeC, until reaching a final concentration of 3% w/v.