In short, these results can be correlated with the increased haemolymph protein, lysozyme, NO, ALP or hepatic CAT and SOD; and the decreased haemolymph AST, ALP or hepatic MDA in treated with the supplement of 0.1e0.2% anthraquinone extract. So the supplement of 0.1e0.2% anthraquinone extract from R. officinale Bail can increase immune and anti-oxidation capability of prawns, enhance resistance to high temperature stress, protect freshwater prawn against the pathogenic infection, and promote the growth of prawns