2.3. FT-IR spectrum analysis and multivariate statistical analysis
Each 20-mg Citrus powder sample in C. unshiu Marc. Cv.
Miyagawa-Wase and the four mutagenized lines were placed in
tubes and mixed with 200 lL methanol. Individual tubes containing extracted samples were incubated in a 50 C water bath for
20 min, and the supernatant was transferred into a new tube after
centrifugation at 10,000g for 15 min. To eliminate cell debris, the
collected supernatant was repeatedly centrifuged and carefully
transferred to a new tube for storage at 20 C prior to undergoing
FT-IR spectroscopy