The isolated S. griseus was grown on
MS with 1 g colloidal chitin. 1ml of the bacterial spore
suspension was inoculated into flasks containing the
production medium and incubated for seven days at 30°C
and at 125 shakes/min. (Remi - C24) At the end of
incubation the culture was the culture was harvested,
filtered and it was centrifuged at 10,000 rpm for 10 min at
4°C and used as crude enzyme. The cell suspension of
S. griseus were re suspended in sterile distilled water at
the rate of 9 x 108 cfu/ml was used to control the fusarium
wilts of tomato [11, 12].