Seeds V. villosa (hairy vetch) were treated with 0.005% colchicine and 12% tetraploid plants
(2n=4x=28) were obtained. Besides two chimeric plants, one of them consisted of diploid, triploid, tetraploid,
hexaploid, heptaploid, octoploid roots and the other diploid and triploid roots were also obtained in hairy
vetch.
Tetraploid plants were determined by the chromosome numbers in the root tips which were confi rmed by studies
conducted in the pollen mother cells (PMCs). Chromosome irregularities in the PMCs of diploids were not
observed, however normal and abnormal chromosomal behavior were detected in tetraploids.
The morphological observations pointed out those tetraploids were shorter, surface of leaves were greater,
and size of stomata were bigger but the number of stomata in per area were less than diploids. Flowering was
delayed in tetraploids, the number of fl owers was less, but forming a few larger fl owers than diploids. It was
also observed that the colour of leaves was darker, and amount of total chlorophyll in the leaves was higher in
tetraploids.
Pollen grains also showed some differences in diploids and tetraploids. While diploid pollen grains had tricolporate,
numbers of pore and colpi showed variations in tetraploids; pollen grains with 4 pores and 4 colpi, 5
pores and 5 colpi and 6 pores and 6 colpi were identifi ed. The pollen size in the tetraploids was defi ned bigger
than others. There were differences in the shapes of pollen grains; they were elliptical in diploids, triangular in
polyploids. Pollen viability was measured as 99% in diploids, and this rate lowered to 81% in tetraploids.
Seeds V. villosa (hairy vetch) were treated with 0.005% colchicine and 12% tetraploid plants(2n=4x=28) were obtained. Besides two chimeric plants, one of them consisted of diploid, triploid, tetraploid,hexaploid, heptaploid, octoploid roots and the other diploid and triploid roots were also obtained in hairyvetch.Tetraploid plants were determined by the chromosome numbers in the root tips which were confi rmed by studiesconducted in the pollen mother cells (PMCs). Chromosome irregularities in the PMCs of diploids were notobserved, however normal and abnormal chromosomal behavior were detected in tetraploids.The morphological observations pointed out those tetraploids were shorter, surface of leaves were greater,and size of stomata were bigger but the number of stomata in per area were less than diploids. Flowering wasdelayed in tetraploids, the number of fl owers was less, but forming a few larger fl owers than diploids. It wasalso observed that the colour of leaves was darker, and amount of total chlorophyll in the leaves was higher intetraploids.Pollen grains also showed some differences in diploids and tetraploids. While diploid pollen grains had tricolporate,numbers of pore and colpi showed variations in tetraploids; pollen grains with 4 pores and 4 colpi, 5pores and 5 colpi and 6 pores and 6 colpi were identifi ed. The pollen size in the tetraploids was defi ned biggerthan others. There were differences in the shapes of pollen grains; they were elliptical in diploids, triangular inpolyploids. Pollen viability was measured as 99% in diploids, and this rate lowered to 81% in tetraploids.
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