2.4. Experimental fish
Five hundred healthy Clownfish, A. sebae (21.4 ± 2.5 g in weight) was collected from the marine ornamental fish hatchery, Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai after checking their health status (Dhayanithi et al., 2012) were stocked in 5000 L cement tank filledwith 2500 L of UV treated seawater; provided
with continuous aeration throughout the experimental period. The fish were acclimatized for 2 weeks prior to initiating the experiment and the fish were fed with 5% of formulated basal control diet (Table 1) corresponding to the body weight twice a day. During the experimental period the water temperature 28.2 ± 1.4 °C, pH 8.2 ± 0.3,
salinity 28 ± 2.2 ppt, dissolved oxygen concentration 5.8 ± 0.6 mg L−1, and 14 h light:10 h dark photoperiod were measured.