Soyasapogenol B is known to have hepatoprotective, antimutagenic, antivirus, and anti-inflammatory
activities. Among five porous carriers tested for immobilization of Aspergillus terreus II for soyasapogenol
B production from soybean saponin, loofa sponge was the best immobilization carrier. Best yield of
83.64% soyasapogenol B was reached with six loofa disks loaded with 107 cells/mL spore concentration
after 72 h in the first cycle. Effective soyasapogenol B yield was also observed using immobilized loofa
disks in successive fermentations up to 8 batches and the highest yield (86.3%) was achieved on the
third batch. Compared to free cell fermentation, soyasapogenol B productivity produced by immobilized
loofa disks was 51.77% higher (64.2 mg/L h vs. 42.3 mg/L h), fermentation time was 33% lower (48 h vs.
72 h) and fermentation medium contents were reduced to half. Immobilization of A. terreus on loofa
sponge, therefore, permits repeated reuse under the specified fermentation conditions for soyasapogenol
B production.
Soyasapogenol B is known to have hepatoprotective, antimutagenic, antivirus, and anti-inflammatory
activities. Among five porous carriers tested for immobilization of Aspergillus terreus II for soyasapogenol
B production from soybean saponin, loofa sponge was the best immobilization carrier. Best yield of
83.64% soyasapogenol B was reached with six loofa disks loaded with 107 cells/mL spore concentration
after 72 h in the first cycle. Effective soyasapogenol B yield was also observed using immobilized loofa
disks in successive fermentations up to 8 batches and the highest yield (86.3%) was achieved on the
third batch. Compared to free cell fermentation, soyasapogenol B productivity produced by immobilized
loofa disks was 51.77% higher (64.2 mg/L h vs. 42.3 mg/L h), fermentation time was 33% lower (48 h vs.
72 h) and fermentation medium contents were reduced to half. Immobilization of A. terreus on loofa
sponge, therefore, permits repeated reuse under the specified fermentation conditions for soyasapogenol
B production.
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