Condition the cartridge with six to ten hold-up volumes of deionized water or weak buffer.
2 Load the sample dissolved in a solution of deionized water or buffer.
3 Elute unwanted weakly bound components with a weak
buffer.
4 Elute the first component of interest with a stronger buffer (change the pH or ionic strength).
5 Elute other components of interest with progressively
stronger buffers.
6 When you recover all of your components, discard the
used cartridge in an appropriate manner.