In vitro propagation techniques were studied in 3 different genotypes of roses (Rosa spp.) and the effects of two
different disinfectants (dichloroisocyanuric acid and sodium hypochlorite) were evaluated. The sterilisation based on
dichloroisocyanuric acid was more efficient (32.6% of vital explants) when compared to the sterilisation with sodium
hypochlorite (7.3% of vital explants). The sterilised and vital explants were transferred onto a shoot proliferation
medium based on MS. The subcultivation period lasted 4 months. From the first genotype, 186 shoots were obtained,
from the second 219 and from the third only 88 shoots. Sufficiently developed plants were transferred to three
different rooting media which differed in the presence of auxin (IBA), sucrose and MS concentration. The results
show, that the rooting medium did not have the same effects on all tested genotypes. The first genotype resulted in
82% rooted plants on the medium with 1/2 MS supplemented with 30 g/L of sucrose and no IBA added. The most
successful rooting for second genotype (55.6% rooted plants) was obtained on the rooting medium with full strength
MS, supplemented with 30 g/L sucrose and 0.5 mg/L IBA. The third genotype rooted most successfully (65.9%
plants with developed roots) on the medium with 1/4 MS supplemented with 30 g/L of sucrose and no IBA added.
Rooted plants were transplanted into the substrate and acclimatised in the laboratory (common growth chamber) and
in the greenhouse (moist chamber). The acclimatisation in the moist chamber had a positive effect on the number of
survived plants (90.9% survival) compared with the acclimatisation in the common growth chamber (68.1% survival).