The content of water in the femur, tibia and L-4 vertebra was
higher by 8.0% (p < 0.05), 11.7% (p < 0.05) and 7.7% respectively in the OVX rats than in the
non-ovariectomized C rats. Administration of glabridin and glycyrrhizic acid to the ovariectomized
rats resulted in decrease of the water content in the femoral bone by 8.2% and 12.1% (p < 0.01)
respectively, as compared to the OVX rats. After treatment with glabridin, the decrease of water
content in the tibia by 16.6% (p < 0.05) was observed, when compared to the OVX group. Similarly,
decrease of the water content (by 12.7%; p < 0.05) in the tibia was noted after treatment with
glycyrrhizic acid, when compared to the OVX rats. In the GL and GA groups the decrease in water
content by 22.1% and 15.7% respectively was observed in the L-4 vertebra when compared to the OVX
group. Administration of estradiol to the ovariectomized rats did not cause any significant
changes in this parameter in all analyzed bones.