We used the PCR–DGGE technique to identify the main microflora in
suan-cai. Compared with traditional methods that rely on isolation and
culture of the organisms present, the PCR–DGGE technique allowed us
to also identify the bacteria and fungi present that cannot be isolated
and cultured in vitro. We were therefore able to make a more comprehensive
measure of the microbial diversity present in suan-cai; this is
important as some of those previously unidentified microbes may be
playing an important role in the development of flavour in suan-cai.