Samples considered significant for diagnostic purposes were collected. Faecal samples and skin scrapings were
examined at the CHV, Dhaka. Blood and urine samples were collected for routine and specific examinations and
were examined at the Central Disease Investigation Laboratory (CDIL), Dhaka. Where needed, owners were
asked to perform X-ray examination from human radiologist to diagnose bone and chest diseases. Dead pet dogs
were subjected to necropsy examination to record the gross lesions and collect samples for laboratory diagnosis.