2.5. Statistical analysis
All experiments and determinations were conducted in triplicates. Presented results are mean values with standard deviations(n = 3). The results were also subjected to one-way analysis of variance (ANOVA) at significance level a = 0.05 using Statistica 10.0 package (StatSoft, Inc., USA). Duncan’s test was used to determine statistically homogenous groups (denoted as subsequent letters of the alphabet) and lowest significant differences (LSD).