Sample Processing
From D0 to D8 (Figure 1), the rats were weighed and urine collected daily using a container on a preservative (Amukin; Gifrer Barbezat, Decines, France). On D8, enteral nutrition was stopped, and the rats were immediately killed (i.e., at the fed state) by decapitation (after anesthesia with isoflurane). This time schedule was selected to assess the effect of the formulas on glycemic control.
Blood.—Blood was sampled in heparin tubes and immediately centrifuged (10 min, 2500 g, 48C). Part of the plasma was deproteinized (with sulfosalicylic acid, 30 mg/mL), and samples were stored at