Extracts of Neem and Tobacco leaves in laboratory
The leaves of Neem and Tobacco were collected from University field. The leaves were washed with distilled water to remove dust and other pollutants and then shelter dry for one week. The dried leaves were crushed with grinder to make fine powder. 50 g of powdered was weighed and then passed to a cellulose extraction tube. Plant materials were extracted by using 250 ml ethanol for 5 hours at (70°C) in a Soxhlet apparatus (250 ml) and the extracts obtained were pour in to the flask separately. The abstract was measured and from each plant materials a final volume of 200 ml was made and pour into the round bottom flasks. The flasks contain plant materials were fitted at the bottom of Rotatory evaporator (Buchi; R-114; Switzerland) separately and evaporated to dryness at a temperature 85°C. The flasks which contain plant dried materials were detached and weighed. Weight of dried extracts was calculated by subtracting the already noted weight of empty flask and few milliliters of ethanol to help in dissolution of extract with water. To the extract add 50 ml of distilled water to get 1g/ml (100% w/v) concentration (Prishanthini, 2014)