and secondly, after the washing, with
the probe requiring the lower stringency conditions. After the
final washing with water, the slides were quickly air-dried with
compressed air. Samples were mounted with the ProLong Gold
antifadent solution (Molecular Probes, Vienna) to avoid the fading
of the fluorescence of the probes. A cover slip was placed on top
and the samples were stored overnight in the dark and then sealed
with nail polish.