Total folate concentration was determined in vegetables by
HPLC with fluorimetric detection after precolumn derivatization,
and expressed as 5-CH3-H4folate in mg/kg of fresh weight.
For spinach, the folate concentration at the beginning of the
experiments, for both pH and the three temperatures studied, varied
slightly between different batches. In all conditions, the folate
concentration first decreased rapidly over time. This decrease
was exponential until reaching a plateau after 60 min (data not
shown). In green beans, the folate concentration at the beginning
of the diffusion time course was the same (about 0.2 mg/kg expressed
in fresh material) for both pH and temperatures studied.
The decrease in folate concentration was much more dependent
on temperature. Thus, at pH 5 and 25 C the folate content was almost
stable. However, it decreased at 45 C and more markedly at
65 C. Folate decrease appeared to be exponential until reaching a
plateau at 120 min, at least at 65 C (data not shown).
Total folate concentration was determined in vegetables byHPLC with fluorimetric detection after precolumn derivatization,and expressed as 5-CH3-H4folate in mg/kg of fresh weight.For spinach, the folate concentration at the beginning of theexperiments, for both pH and the three temperatures studied, variedslightly between different batches. In all conditions, the folateconcentration first decreased rapidly over time. This decreasewas exponential until reaching a plateau after 60 min (data notshown). In green beans, the folate concentration at the beginningof the diffusion time course was the same (about 0.2 mg/kg expressedin fresh material) for both pH and temperatures studied.The decrease in folate concentration was much more dependenton temperature. Thus, at pH 5 and 25 C the folate content was almoststable. However, it decreased at 45 C and more markedly at65 C. Folate decrease appeared to be exponential until reaching aplateau at 120 min, at least at 65 C (data not shown).
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