he aim of this study was to investigate the effect of diets supplemented with fish oil, flaxseed, and chiaseed on the omega-3 fatty acid composition and sensory properties of hens’ eggs. No significant difference in yolk fatcontent was found between treatments. The fatty acid composition of egg yolk was significantly affected by the dietarytreatments. Inclusion of chia at 300 g/kg into the diet produced eggs with the highest concentration of omega-3 fatty acid.Eicosapentaenoic acid and docosahexaenoic acid were only detected in eggs from laying hens fed the diet supplementedwith fish oil. Diet had a significant effect on color, flavor and overall acceptability of eggs. Types and levels of omega-3fatty acids in feed influence the level of yolk omega-3 fatty acids in egg yolk. Inclusion of chia into the hens’ dietsignificantly increased the concentration of yolk omega-3 fatty acid without significant change in sensory properties.