On the last day of each experimental period, blood samples (10 mL)were taken 4 h after feeding from the jugular vein of each goat into a clean dry tube, without anticoagulants.
Blood samples were centrifugedat 4000 × g at 4◦C for 20 min.
Serum was separated into 2 mL clean dried Eppendorf tubes and frozen at −20◦C until analysis.
Blood serum samples were analyzed for concentrations of total protein, albumin, urea-N, glutamate-oxaloacetate transaminase (GOT),glutamate-pyruvate transaminase (GPT), glucose, creatinine, cholesteroland triglycerides using specific kits (Stanbio Laboratory, Boerne, Texas,USA) following manufacturer instructions. Globulin concentration was calculated.