The aim of this study was to investigate petroleum phytoremediation enhancement by plant growth
promoting bacteria (PGPR), specifically the correlation between petroleum hydrocarbon fractions and
bacterial community structure affected by remediation and PGPR inocula. Aged petroleum contaminated
soil was remediated by tall fescue (Testuca arundinacea L.) inoculated with two PGPR strains.
Hydrocarbon degradation was measured by GC–MS (Gas-chromatography Mass-spectrometer) based
on carbon fraction numbers (C8–C34). Changes in bacterial community structure were analyzed by
high-throughput pyrosequencing of 16s rRNA. PGPR inoculation increased tall fescue biomass and
petroleum hydrocarbons were removed in all the treatments. Maximum hydrocarbon removal, particular
high molecular weight (C21–C34) aliphatic hydrocarbons (AHs) and polycyclic aromatic hydrocarbons
(PAHs), was observed in tall fescue inoculated with PGPR. The relative abundance of phyla
c-proteobacteria and Bacteroidetes increased after different treatments compared with controls.
Moreover, a bacterial guild mainly comprising the genera Lysobacter, Pseudoxanthomonas, Planctomyces,
Nocardioides, Hydrogenophaga, Ohtaekwangia was found to be positively correlated with C21–C34
petroleum hydrocarbons fractions removal by RDA analysis, implying that petroleum degradation was
unrelated to bacterial community diversity but positively correlated with specific petroleum degraders
and biosurfactant producers.
The aim of this study was to investigate petroleum phytoremediation enhancement by plant growthpromoting bacteria (PGPR), specifically the correlation between petroleum hydrocarbon fractions andbacterial community structure affected by remediation and PGPR inocula. Aged petroleum contaminatedsoil was remediated by tall fescue (Testuca arundinacea L.) inoculated with two PGPR strains.Hydrocarbon degradation was measured by GC–MS (Gas-chromatography Mass-spectrometer) basedon carbon fraction numbers (C8–C34). Changes in bacterial community structure were analyzed byhigh-throughput pyrosequencing of 16s rRNA. PGPR inoculation increased tall fescue biomass andpetroleum hydrocarbons were removed in all the treatments. Maximum hydrocarbon removal, particularhigh molecular weight (C21–C34) aliphatic hydrocarbons (AHs) and polycyclic aromatic hydrocarbons(PAHs), was observed in tall fescue inoculated with PGPR. The relative abundance of phylac-proteobacteria and Bacteroidetes increased after different treatments compared with controls.Moreover, a bacterial guild mainly comprising the genera Lysobacter, Pseudoxanthomonas, Planctomyces,Nocardioides, Hydrogenophaga, Ohtaekwangia was found to be positively correlated with C21–C34petroleum hydrocarbons fractions removal by RDA analysis, implying that petroleum degradation wasunrelated to bacterial community diversity but positively correlated with specific petroleum degradersand biosurfactant producers.
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