Based on the PLS-DA analysis, the C. unshiu Marc. Cv.
Miyagawa-Wase and M2 lines had the closest phylogenic
relationship, and the M1 and M3 lines were closer with regard to
characteristic traits. However, M4 lines, which were separately
grouped from the control line, had a similar phylogenetic relationship pattern in both PCA and PLS-DA analyses. It was a relatively
low phylogenic relationship of characteristic trait. This result
showed that the mutation induced a different phenotype;