Two experiments testing the ammonia-lowering capacity of the
strains in soaking solution were carried out under different conditions.
In the first experiment, two yeast strains (S. cerevisiae
Misaki-1 and the selected isolate) and two LAB strains (L. plantarum
Sanriku-SU1 and the selected isolate) were incubated in 40 mL of
GYP and MRS broths, respectively, at 30 C for 2 days. Then, the
yeast and LAB culture cells were suspended and adjusted to
OD600 ¼ 4.5 (1.5 of yeast and 3.0 of LAB) in 6% sucrose and 6% NaCl
solution. Five grams of fresh salmon shark meat (an approximately
1.5e2.0 cm cube) was soaked in 10 mL of the cell suspension in a
50 mL tube (Polypropylene; TPP, Trasadingen, Switzerland) and
incubated at 10 C for 2 days. From the results of these experiments,
one strain each of yeast and LAB was selected. In the second
experiment, 50 g of salmon meat was soaked in 100 mL of 100 g/L