Pullulan (Pul) was supplied by Hayashibara Company (Okayama,
Japan). Glycerin (Gly) was obtained from VWR Company (Batavia IL,
USA); xanthan gum (Xa)was purchased from TCI America (Portland
OR, USA), and locust bean (Lb) from CP Kelco (Lille Skensved,
Denmark).
2.3. Film formation
Films were made following the instructions provided by the
manufacturer with some modifications. Briefly, different aqueous
solutions of Pul (20e220 g/l) were prepared by stirring the powder
in distilled water (100 ml final volume) for 10 min; afterward
various concentrations of Gly (5e65 g/l), to plasticize the films, and
Xa and Lb (1e5 g/l), to stabilize the matrix solution, were added in
sequence. When all the components were completely dissolved
(w20 min), the solution was autoclaved at 121 C for 15 min. Films
were cast by pipetting 10 ml of solution into sterile plastic plates
with inner diameters of 10 cm and allowed to dry for 24 h at 25 C
and 40% relative humidity (RH).
Pullulan (Pul) was supplied by Hayashibara Company (Okayama,
Japan). Glycerin (Gly) was obtained from VWR Company (Batavia IL,
USA); xanthan gum (Xa)was purchased from TCI America (Portland
OR, USA), and locust bean (Lb) from CP Kelco (Lille Skensved,
Denmark).
2.3. Film formation
Films were made following the instructions provided by the
manufacturer with some modifications. Briefly, different aqueous
solutions of Pul (20e220 g/l) were prepared by stirring the powder
in distilled water (100 ml final volume) for 10 min; afterward
various concentrations of Gly (5e65 g/l), to plasticize the films, and
Xa and Lb (1e5 g/l), to stabilize the matrix solution, were added in
sequence. When all the components were completely dissolved
(w20 min), the solution was autoclaved at 121 C for 15 min. Films
were cast by pipetting 10 ml of solution into sterile plastic plates
with inner diameters of 10 cm and allowed to dry for 24 h at 25 C
and 40% relative humidity (RH).
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