In-house testing at several independent forensic laboratories has determined that no other forensically relevant body fluid (sweat, semen and vaginal secretion) will react within 10 minutes using the current protocol, even after repeated deposition. The exception is faecal stains that may contain levels of amylase as high as those found in saliva. For this reason positive observations within areas obviously contaminated with faeces should not be interpreted for the presence of saliva. The presence of potential faecal material on an item should be recorded in the examination notes. In an independent trial performed in UK it was demonstrated that saliva from the most common pets did not give rise to any false positives.