and the supernatant
was heated at 80 C for 10 min and then filter-sterilized (0.22 mm
pore size filter-units, Millipore). Aliquots of one milliliter of the cell
suspensions and one milliliter of the bacteriocin preparation (or
sterile water for the negative control) were homogenized and the
number of viable cells was determined before and after incubation
during