Fatty acid methyl esters (FAME) were analyzed using a flame ionization gas chromatograph
(Shimadzu GC-2014) equipped with an Omegawax 250 capillary column (30 m L X
0.25 mm internal diameter), a flame ionization detector (FID) and a split injection system
with nitrogen carrier gas. The injector port and detector temperatures were maintained at
250 and 260 C, respectively. The column temperature program was held at 140 C for
5 min and then elevated at a rate of 3 C/min to 200 C. Total run time was 60 min per
sample. Fatty acids were identified by comparing their retention times to that of fatty acid
standards (Sigma-Aldrich Co, USA).