A total of 56 accessions of Kundang (B. macrophylla) and 23
accessions of Remia (B. oppositifolia) were collected from
Peninsular Malaysia and used for DNA using protocol based on
Murray and Thompson (1980) with some minor modifications where
the mixture between dry tissue powder and extraction buffer was
incubated for 2 h at 65°C with occasional gentle mixing.
Polymerase