tIn this study, we have investigated the colorimetric sensing of heavy metal ions, especially copper ions(Cu2+) for multiple analyses using gelatin functionalized silver nanoparticles (AgNPs) and AgNPs loadedgelatin hydrogels. Gelatin functionalized AgNPs were prepared by chemical reduction of silver nitrate.The gelatin functionalized AgNPs showed concentration dependent color variation (yellow to green color)in the range from 1 × 10−8to 1 × 10−4M with lower detection limit of 1 × 10−7M under optimal conditionon interaction with Cu2+ions. The selectivity response of AgNPs to various metal ions has been evaluatedand found that the present method is highly selective for Cu2+owing to agglomeration of gelatin func-tionalized AgNPs and formation of complex between Cu2+and amino group of gelatin. Similarly, studieshave been carried out using AgNPs loaded gelatin hydrogels for the detection of Cu2+. The regenerationof AgNPs and hydrogels was carried out by using ethylenediaminetetraacetic acid (EDTA). The sensorresponse of real sample was evaluated with pond water and the sensitivity values are in good agreementwith the value obtained by atomic absorption spectroscopy (AAS) analysis. To the best of our knowledge,the proposed sensor system is new and shows promising result for simple and low-cost sensing of Cu2+which may be explored for pollution monitoring applications